Steve Borthwick has made some big calls ahead of England's Nations Championship campaign, leaving several players from his previous squads out of his latest 36-man training group.

England face Australia, Japan and New Zealand next month, but several familiar faces have missed the cut.

Ben Spencer, Theo Dan, Ted Hill, Charlie Ewels, Vilikesa Sela and George Kloska are all absent, although injuries and competition for places have played different roles in their situations.

There is better news for Joe Marchant, Tom Pearson and Will Joseph, who return to the England setup.

Meanwhile, three uncapped players have been handed opportunities to impress.

Here are six players who have missed out.

Six England stars left out

1. Theo Dan (Saracens)

Perhaps the biggest surprise.

The Saracens hooker has been a regular part of Borthwick's plans, but there is no room for him this time.

Jamie George and Luke Cowan-Dickie are joined by exciting Bath youngster Kepu Tuipulotu.

As RUCK reported in its full England squad announcement, Tuipulotu's inclusion comes at Dan's expense.

2. Ben Spencer (Bath Rugby)

The experienced scrum-half is also absent from the latest squad.

Spencer has been recovering from shoulder surgery, making his situation different from a straightforward selection omission.

England have named Alex Mitchell and Jack van Poortvliet, while Northampton's Archie McParland has also earned his first senior call-up.

3. Ted Hill (Bath Rugby)

Hill will be disappointed to miss out after featuring in England's summer plans.

The Bath forward has plenty of competition, with Ben Earl, Henry Pollock, Tom Curry and Ben Curry all included.

Tom Pearson's return has added another option to an already crowded back row.

4. Charlie Ewels (Bath Rugby)

The experienced lock is another player missing from Borthwick's latest group.

Maro Itoje returns after missing the summer campaign, while Ollie Chessum, George Martin, Alex Coles and Arthur Clark are also selected.

Ewels now faces a battle to force his way back into contention.

5. Vilikesa Sela (Bath Rugby)

The young prop was part of England's summer squad but has not made the latest group.

Will Stuart returns, with Joe Heyes and Asher Opoku-Fordjour also competing for places at tighthead.

Sela still has plenty of time on his side.

6. George Kloska (Bristol Bears)

The Bristol Bears front-rower completes the list.

Kloska was included in England's September training squad but has not retained his place.

He will hope strong club performances can put him back in the conversation.